qubit broad range dna 3 0 fluorometer kit Search Results


tm dna 30 end biotinylation kit  (Thermo Fisher)
99
Thermo Fisher tm dna 30 end biotinylation kit
Tm Dna 30 End Biotinylation Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/qubit+broad+range+dna+3+0+fluorometer+kit/pm28419348-287-13-19?v=Thermo+Fisher
Average 99 stars, based on 1 article reviews
tm dna 30 end biotinylation kit - by Bioz Stars, 2026-08
99/100 stars
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sir dna  (Cytoskeleton Inc)
96
Cytoskeleton Inc sir dna
A , Representative immunofluorescence images of METs in RAW264.7 macrophage cell line. Two h after stimulation with mitochondrial DNA (mtDNA, 250 ng/mL) or nuclear DNA (nDNA, 250 ng/mL), cells were stained with an anti-citrullinated histone H3 antibody (CitH3, magenta) and DAPI (blue). Scale bars, 50 μm. B , Quantitative analysis of MET formation. The percentage of CitH3 + -positive cells are shown (n=3). C , Representative immunofluorescence image of METs after PAD4 inhibition. Thirty min after pretreatment with the PAD4 inhibitor GSK484 at the indicated concentrations or vehicle, RAW264.7 cells were exposed to mtDNA for 2 h and stained with an anti-CitH3 antibody (magenta) and DAPI (blue). Scale bars, 50 μm. D , Quantitative analysis of MET formation (n=3). E , Time-lapse imaging of bone marrow-derived macrophages (BMDMs) from wild-type (WT) or PAD4 knockout (PAD4KO) mice following mtDNA <t>stimulation.</t> <t>SiR-DNA</t> (magenta) was used to visualize nuclei and the cell-impermeable SYTOX dye (green) was used to detect extracellular DNA. Representative images at 0, 160 and 200 min are shown. Images at higher magnification are displayed from boxed area in the lower panels. Scale bars, 50 μm. F , Quantification of SYTOX-positive cells (n=4). *P < 0.05 by two-way repeated-measures analysis of variance followed by Sidak’s multiple comparisons. G , WT or PAD4KO BMDMs were stimulated with mtDNA for 6 h, fixed, and stained with anti-CD68 (green) and anti-CitH3 (magenta) antibodies and DAPI (blue). Representative immunofluorescence images are shown. Arrowheads indicate CitH3-positive METs. H , Quantification of MET formation (n=3). *P < 0.05 by one-way analysis of variance with Tukey’s post hoc analysis. All data are presented as mean ± SEM.
Sir Dna, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/qubit+broad+range+dna+3+0+fluorometer+kit/bio_rxiv__64898__2026__03__15__711858-85-4-10?v=Cytoskeleton+Inc
Average 96 stars, based on 1 article reviews
sir dna - by Bioz Stars, 2026-08
96/100 stars
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dna samples  (Cytiva Europe)
94
Cytiva Europe dna samples
A , Representative immunofluorescence images of METs in RAW264.7 macrophage cell line. Two h after stimulation with mitochondrial DNA (mtDNA, 250 ng/mL) or nuclear DNA (nDNA, 250 ng/mL), cells were stained with an anti-citrullinated histone H3 antibody (CitH3, magenta) and DAPI (blue). Scale bars, 50 μm. B , Quantitative analysis of MET formation. The percentage of CitH3 + -positive cells are shown (n=3). C , Representative immunofluorescence image of METs after PAD4 inhibition. Thirty min after pretreatment with the PAD4 inhibitor GSK484 at the indicated concentrations or vehicle, RAW264.7 cells were exposed to mtDNA for 2 h and stained with an anti-CitH3 antibody (magenta) and DAPI (blue). Scale bars, 50 μm. D , Quantitative analysis of MET formation (n=3). E , Time-lapse imaging of bone marrow-derived macrophages (BMDMs) from wild-type (WT) or PAD4 knockout (PAD4KO) mice following mtDNA <t>stimulation.</t> <t>SiR-DNA</t> (magenta) was used to visualize nuclei and the cell-impermeable SYTOX dye (green) was used to detect extracellular DNA. Representative images at 0, 160 and 200 min are shown. Images at higher magnification are displayed from boxed area in the lower panels. Scale bars, 50 μm. F , Quantification of SYTOX-positive cells (n=4). *P < 0.05 by two-way repeated-measures analysis of variance followed by Sidak’s multiple comparisons. G , WT or PAD4KO BMDMs were stimulated with mtDNA for 6 h, fixed, and stained with anti-CD68 (green) and anti-CitH3 (magenta) antibodies and DAPI (blue). Representative immunofluorescence images are shown. Arrowheads indicate CitH3-positive METs. H , Quantification of MET formation (n=3). *P < 0.05 by one-way analysis of variance with Tukey’s post hoc analysis. All data are presented as mean ± SEM.
Dna Samples, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/qubit+broad+range+dna+3+0+fluorometer+kit/pm18474553-59-1-37?v=Cytiva+Europe
Average 94 stars, based on 1 article reviews
dna samples - by Bioz Stars, 2026-08
94/100 stars
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bdna signal amplification assay kit  (Siemens Healthineers)
86
Siemens Healthineers bdna signal amplification assay kit
A , Representative immunofluorescence images of METs in RAW264.7 macrophage cell line. Two h after stimulation with mitochondrial DNA (mtDNA, 250 ng/mL) or nuclear DNA (nDNA, 250 ng/mL), cells were stained with an anti-citrullinated histone H3 antibody (CitH3, magenta) and DAPI (blue). Scale bars, 50 μm. B , Quantitative analysis of MET formation. The percentage of CitH3 + -positive cells are shown (n=3). C , Representative immunofluorescence image of METs after PAD4 inhibition. Thirty min after pretreatment with the PAD4 inhibitor GSK484 at the indicated concentrations or vehicle, RAW264.7 cells were exposed to mtDNA for 2 h and stained with an anti-CitH3 antibody (magenta) and DAPI (blue). Scale bars, 50 μm. D , Quantitative analysis of MET formation (n=3). E , Time-lapse imaging of bone marrow-derived macrophages (BMDMs) from wild-type (WT) or PAD4 knockout (PAD4KO) mice following mtDNA <t>stimulation.</t> <t>SiR-DNA</t> (magenta) was used to visualize nuclei and the cell-impermeable SYTOX dye (green) was used to detect extracellular DNA. Representative images at 0, 160 and 200 min are shown. Images at higher magnification are displayed from boxed area in the lower panels. Scale bars, 50 μm. F , Quantification of SYTOX-positive cells (n=4). *P < 0.05 by two-way repeated-measures analysis of variance followed by Sidak’s multiple comparisons. G , WT or PAD4KO BMDMs were stimulated with mtDNA for 6 h, fixed, and stained with anti-CD68 (green) and anti-CitH3 (magenta) antibodies and DAPI (blue). Representative immunofluorescence images are shown. Arrowheads indicate CitH3-positive METs. H , Quantification of MET formation (n=3). *P < 0.05 by one-way analysis of variance with Tukey’s post hoc analysis. All data are presented as mean ± SEM.
Bdna Signal Amplification Assay Kit, supplied by Siemens Healthineers, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/qubit+broad+range+dna+3+0+fluorometer+kit/pm26201776-58-26-35?v=Siemens+Healthineers
Average 86 stars, based on 1 article reviews
bdna signal amplification assay kit - by Bioz Stars, 2026-08
86/100 stars
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Image Search Results


A , Representative immunofluorescence images of METs in RAW264.7 macrophage cell line. Two h after stimulation with mitochondrial DNA (mtDNA, 250 ng/mL) or nuclear DNA (nDNA, 250 ng/mL), cells were stained with an anti-citrullinated histone H3 antibody (CitH3, magenta) and DAPI (blue). Scale bars, 50 μm. B , Quantitative analysis of MET formation. The percentage of CitH3 + -positive cells are shown (n=3). C , Representative immunofluorescence image of METs after PAD4 inhibition. Thirty min after pretreatment with the PAD4 inhibitor GSK484 at the indicated concentrations or vehicle, RAW264.7 cells were exposed to mtDNA for 2 h and stained with an anti-CitH3 antibody (magenta) and DAPI (blue). Scale bars, 50 μm. D , Quantitative analysis of MET formation (n=3). E , Time-lapse imaging of bone marrow-derived macrophages (BMDMs) from wild-type (WT) or PAD4 knockout (PAD4KO) mice following mtDNA stimulation. SiR-DNA (magenta) was used to visualize nuclei and the cell-impermeable SYTOX dye (green) was used to detect extracellular DNA. Representative images at 0, 160 and 200 min are shown. Images at higher magnification are displayed from boxed area in the lower panels. Scale bars, 50 μm. F , Quantification of SYTOX-positive cells (n=4). *P < 0.05 by two-way repeated-measures analysis of variance followed by Sidak’s multiple comparisons. G , WT or PAD4KO BMDMs were stimulated with mtDNA for 6 h, fixed, and stained with anti-CD68 (green) and anti-CitH3 (magenta) antibodies and DAPI (blue). Representative immunofluorescence images are shown. Arrowheads indicate CitH3-positive METs. H , Quantification of MET formation (n=3). *P < 0.05 by one-way analysis of variance with Tukey’s post hoc analysis. All data are presented as mean ± SEM.

Journal: bioRxiv

Article Title: Macrophage extracellular traps promote maladaptive cardiac remodelling and heart failure via PAD4-dependent mechanisms

doi: 10.64898/2026.03.15.711858

Figure Lengend Snippet: A , Representative immunofluorescence images of METs in RAW264.7 macrophage cell line. Two h after stimulation with mitochondrial DNA (mtDNA, 250 ng/mL) or nuclear DNA (nDNA, 250 ng/mL), cells were stained with an anti-citrullinated histone H3 antibody (CitH3, magenta) and DAPI (blue). Scale bars, 50 μm. B , Quantitative analysis of MET formation. The percentage of CitH3 + -positive cells are shown (n=3). C , Representative immunofluorescence image of METs after PAD4 inhibition. Thirty min after pretreatment with the PAD4 inhibitor GSK484 at the indicated concentrations or vehicle, RAW264.7 cells were exposed to mtDNA for 2 h and stained with an anti-CitH3 antibody (magenta) and DAPI (blue). Scale bars, 50 μm. D , Quantitative analysis of MET formation (n=3). E , Time-lapse imaging of bone marrow-derived macrophages (BMDMs) from wild-type (WT) or PAD4 knockout (PAD4KO) mice following mtDNA stimulation. SiR-DNA (magenta) was used to visualize nuclei and the cell-impermeable SYTOX dye (green) was used to detect extracellular DNA. Representative images at 0, 160 and 200 min are shown. Images at higher magnification are displayed from boxed area in the lower panels. Scale bars, 50 μm. F , Quantification of SYTOX-positive cells (n=4). *P < 0.05 by two-way repeated-measures analysis of variance followed by Sidak’s multiple comparisons. G , WT or PAD4KO BMDMs were stimulated with mtDNA for 6 h, fixed, and stained with anti-CD68 (green) and anti-CitH3 (magenta) antibodies and DAPI (blue). Representative immunofluorescence images are shown. Arrowheads indicate CitH3-positive METs. H , Quantification of MET formation (n=3). *P < 0.05 by one-way analysis of variance with Tukey’s post hoc analysis. All data are presented as mean ± SEM.

Article Snippet: Cells were incubated with SiR-DNA (30 min at 1 μM, Cytoskeleton, CY-SC007) and SYTOX Green (Thermo Fisher Scientific) according to the manufacturers’ protocols.

Techniques: Immunofluorescence, Staining, Inhibition, Imaging, Derivative Assay, Knock-Out